Reporter

Part:BBa_K5194008:Design

Designed by: Nirmal Cheema   Group: iGEM24_UCalgary   (2024-09-26)


pD-MSP1D1 insert


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 117
    Illegal XbaI site found at 176
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 117
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 117
    Illegal BglII site found at 173
    Illegal BglII site found at 545
    Illegal XhoI site found at 441
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 117
    Illegal XbaI site found at 176
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 117
    Illegal XbaI site found at 176
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The plasmid was designed by Stephen Sligar.


Source

pMSP1D1 was a gift from Stephen Sligar (Addgene plasmid # 20061 ; http://n2t.net/addgene:20061 ; RRID:Addgene_20061) https://www.addgene.org/20061/

References